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Infrared spectroscopy is probably the most common spectroscopic technique employed by chemists today and in particular analytical chemists. There are a number of reasons for its popularity. It can be used in qualitative analysis to identify specific substances for which there are reference spectra, it can be used to aid in structure elucidation and, in particular identify specific chemical groups present in a compound. In certain instances it can also be employed for quantitative analysis.
In addition, the infrared spectrometer is relatively inexpensive, compact in size, so it can stand on a laboratory bench without taking up too much room, is easy to operate and produces spectra in a few minutes or less. The technique is not as sensitive as UV/vis or mass spectrometry but its sensitivity is more than adequate for most analytical purposes.
In practice, whereas UV/Visible spectra are usually represented as curves relating transmission or absorbance against wavelength, in infrared spectroscopy the independent variable is usually measured as wave numbers, as opposed to wavelength.
The wave number for a particular wave having frequency (ν) is taken as (1/λ) which is the number of waves per centimeter.
Bearing in mind that E =h.ν and c = ν.λ,
where (E) is the energy of the photon, (ν) is the frequency of the radiation, (λ) is the wavelength of the radiation, and (h) is Planks Constant.
If (Δ) is the wave number of a photon of wavelength (λ)
Then Δ = (1/λ) = (ν/c) = E/hc
and is expressed in reciprocal centimetres (cm -1).
Both forms of Spectrum presentation (i.e. curves relating absorption against wavelength and wave number) are shown in Figure 2 and it is seen that the appearance of each Spectrum by the two methods is very different.
The infrared band of frequencies can be divided roughly into three groups of interest to the analyst or practicing chemist. The three groups are given in the following table. Of the three groups the group dealing with fundamental rotational-vibration energies have, so far, be found the most useful to the practical chemist.
|Region||Wavenumber Range||wavelength Range|
|Near Infra Red (overtones||13,300 - 4000||0.75 - 2.5|
|Fundamental Rotation Vibration||4000 - 400||2.5 - 25|
|Far Infrared (skeletal vibrations)||400 - 20||26-500|
spectroscopy has been shown to be a particularly valuable aid to the
organic chemists. Organic substances exhibit explicit absorption
characteristics to radiation of specific frequencies that can be
exclusive for a particular substance. In addition, the Spectrum of a
mixture usually constitutes a Spectrum addition in that it contains
the spectra of both substances superimposed. Finally the intensity of
the absorption at any particular wavelength is related to the
quantity of the substance present in the sample.
About the Author
RAYMOND PETER WILLIAM SCOTT was born on June 20 1924 in Erith, Kent, UK. He studied at the University of London, obtaining his B.Sc. degree in 1946 and his D.Sc. degree in 1960. After spending more than a decade at Benzole Producers, Ltd. Where he became head of the Physical Chemistry Laboratory, he moved to Unilever Research Laboratories as Manager of their Physical Chemistry department. In 1969 he became Director of Physical Chemistry at Hoffmann-La Roche, Nutley, NJ, U.S.A. and subsequently accepted the position of Director of the Applied Research Department at the Perkin-Elmer Corporation, Norwalk, CT, U.S.A.
In 1986 he became an independent consultant and was appointed Visiting Professor at Georgetown
University, Washington, DC, U.S.A. and at Berkbeck College of the University of London; in 1986 he retired but continues to write technical books dealing with various aspects of physical chemistry and physical chemical techniques. Dr. Scott has authored or co-authored over 200 peer reviewed scientific papers and authored, co-authored or edited over thirty books on various aspects of physical and analytical chemistry. Dr. Scott was a founding member of the British chromatography Society and received the American Chemical society Award in chromatography (1977), the M. S. Tswett chromatography Medal (1978), the Tswett chromatography Medal U.S.S.R., (1979), the A. J. P. Martin chromatography Award (1982) and the Royal Society of Chemistry Award in Analysis and Instrumentation (1988).
Dr. Scott’s activities in gas chromatography started at the inception of the technique, inventing the Heat of Combustion Detector (the precursor of the Flame Ionization Detector), pioneered work on high sensitivity detectors, high efficiency columns and presented fundamental treatments of the relationship between the theory and practice of the technique. He established the viability of the moving bed continuous preparative gas chromatography, examined both theoretically and experimentally those factors that controlled dispersion in packed beds and helped establish the gas chromatograph as a process monitoring instrument. Dr. Scott took and active part in the renaissance of liquid chromatography, was involved in the development of high performance liquid chromatography and invented the wire transport detector. He invented the liquid chromatography mass spectrometry transport interface, introduced micro-bore liquid chromatography columns and used them to provide columns of 750,000 theoretical plates and liquid chromatography separations in less than a second. Dr. Scott has always been a “hands-on” scientist with a remarkable record of accomplishments in chromatography ranging from hardware design to the development of fundamental theory. He has never shied away from questioning “conventional wisdom” and his original approach to problems has often produced significant breakthroughs.